A novel electrochemical assay for chymosin determination using a label-free peptide as a substrate

Chymosin is a predominant enzyme in rennet and used cheese production because of its excellent milk-clotting activity. Herein, we proposed facile label-free electrochemical method for determining chymosin activity based on peptide-based substrate. The synthesized substrate peptide was assembled onto the surface Au-deposited grassy carbon electrode. current proportional to activity, thus could be determined. detection ranges were 2.5 25 U mL−1. limit 0.8 sensing platform quantify commercial with high selectivity, stability, satisfactory reproducibility. We developed facile, fast, effective assay detecting which has potential applications cheesemaking.